Salivary TAS, TOS, LL-37 and Dental Status in Passive Smoking Children

NCT04292548 | Completed

• 1 other identifier: Kırıkkale pediatric dentistry
• Study Type: observational
• Target: 180
• Locations: 0 countries
• Sites: N/A

Brief Summary

The aim of the present study was to determine the effect of passive smoking on the levels of salivary TAS, TOS, LL-37 and dental status in children.In the study the parents of children were asked to fill out a complete survey about smoking habits. After filling out the questionnaire, according to the survey results, a total of 180 children were included to the study as follows; 90 children exposed to passive smoking, and 90 children in the control group (unexposed controls). Also demographic data were recorded (age, gender, parental education levels, child's tooth-brushing habit and child's daily dietary sugar exposure, family income). Dental examination of children were performed and caries prevalance of the patients were recorded. Unstimulated saliva samples were collected from children. Saliva 'cotinine levels' which are expected to increase in passive smoking group; 'antimicrobial peptide LL-37' and oxidative stress markers 'total antioxidant status' (TAS), and' total oxidant status' (TOS) were evaluated by using Enzyme-linked immunosorbent assay (ELISA). The obtained data parameters of the two groups were evaluated and comparison was performed.

Conditions

Passive Smoking; Oxidative Stress; Dental Caries; Children

Outcome Measures

Primary Outcomes (4)

• salivary oxidative stress values: TAS level

  Unstimulated saliva samples were collected from children.

  1 day (once after the dental examination)

• salivary oxidative stress values: TOS level

  Unstimulated saliva samples were collected from children.

  1 day (once after the dental examination)

• salivary LL-37 level

  Unstimulated saliva samples were collected from children.

  1 day (once after the dental examination)

• salivary cotinine levels

  Unstimulated saliva samples were collected from children for describing passive smoking children.

  1 day (once after the dental examination)

Study Arms (2)

study group (passive smoking children)

Diagnostic Test: taking saliva samples to determine TAS, TOS and LL-37

control group

Diagnostic Test: taking saliva samples to determine TAS, TOS and LL-37

Interventions

taking saliva samples to determine TAS, TOS and LL-37 DIAGNOSTIC_TEST

control group; study group (passive smoking children)

Eligibility Criteria

• Age: 6 Years - 12 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)
• Sampling Method: Non-Probability Sample

Study Population

Children, referring for dental examination to the Department of Pediatric Dentistry of Kirikkale University, were selected.

You may qualify if:

• Systemically healthy children

You may not qualify if:

• The presence of orthodontic / intraoral appliance
• Any disease or condition known to affect defense system
• Uncooperative children

Sponsors & Collaborators

• Merve Erkmen Almaz: lead
• Kırıkkale University: collaborator

MeSH Terms

Conditions

Dental Caries

Condition Hierarchy (Ancestors)

Tooth Demineralization; Tooth Diseases; Stomatognathic Diseases

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Assist prof

Study Record Dates

• First Submitted: February 28, 2020
• First Posted: March 3, 2020
• Study Start: November 16, 2016
• Primary Completion: April 15, 2018
• Study Completion: August 15, 2018
• Last Updated: March 3, 2020

Record last verified: 2020-02

Data Sharing

• IPD Sharing: Will not share